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ELISA - Protein Scientist

Job Title: ELISA - Protein Scientist
Contract Type: Permanent
Location: Cambridge, Cambridgeshire
Industry:
Salary: Negotiable
Reference: J896338_1533830662
Contact Name: Tom Hammond
Contact Email: tom.hammond@russell-taylor.co.uk
Job Published: August 09, 2018 17:04

Job Description

ELISA - Protein Scientist

Location: Cambridge

Type: Permanent

Hours: Days

Our Client, a leading multi - sited Life Sciences company are looking to recruit and experienced Protein Scientist.

The Role:

  • Clone, express, purify and characterise proteins for protein characterisation work, binding studies and stability testing using a range of different expression platforms (bacterial, Pichia or mammalian cells)
  • Clone, express, purify and characterise proteins to be used in phage screens and ELISA/cell based assays from a range of expression platforms and fully characterise them using a range of methods (e.g. HPLC, SDS-PAGE, IEF and SPR).
  • Maintain and establish cell lines required for protein production
  • Carry out optimisation studies to increase production from microbial hosts
  • Develop bench scale protein purification protocols using conventional chromatography resins to purify gram quantities of proteins
  • Carry out HPLC based assays to characterise protein batches produced in-house (e.g. size exclusion, reverse phase chromatography etc.)
  • Carry out accelerated stability studies on in house proteins and characterise changes that may occur
  • Establish formulation studies for increasing stability of the proteins produced
  • Produce high quality and tool molecules for pre-clinical studies, delivering within timelines at the correct specification
  • Carry out R&D projects to improve the platform for therapeutic development such as improving the biophysical properties of the scaffold

The Person:

  • Will have a A BSc/MSc in protein biochemistry, protein engineering or molecular biology, any laboratory experience would be an advantage.
  • Standard DNA manipulation/cloning techniques and protein engineering including working with a range of protein expression platforms (bacterial, Pichia and mammalian)
  • Practical knowledge of the AKTA purification platform, Unicorn software and using conventional chromatography resins e.g. ion exchange
  • Developing HPLC based assays for protein characterisation
  • Excellent knowledge of a range of methodologies to characterise proteins (e.g. SPR, ELISA, Western blot)
  • A good understanding of protein engineering and protein biophysics to investigate structure/functional relationships would be an advantage.

Important Information: We endeavour to process your personal data in a fair and transparent manner. In applying for this role, Russell Taylor will be acting within your interest and will contact you in relation to the role, either by email, phone or text message. For more information see our Privacy Policy h t t p s : / / w w w . r u s s e l l - t a y l o r . c o . u k / p r i v a c y - p o l i c y on our website. It is important you are aware of your individual rights and the provisions the company has put in place to protect your data. If you would like further information on the policy or the GDPR please contact us on h r @ r u s s e l l - t a y l o r . c o . u k

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